Standard protocols suggest 5% vol/vol. We have had great luck at that concentration. There is one big difference between PLTMax® and FBS: You CAN use too much PLTMax®. It will slow growth of MSC at high concentrations. But feel free to titrate it to maximize the growth of your cells. We have reports of different concentrations required with different base medias. We are working on a report to help guide users that will be released soon.